Journal:

Article Title: A protocol for a lung neovascularization model in rodents

doi: 10.1038/nprot.2007.536

Figure Lengend Snippet: Drawing illustrating the inflation apparatus to distend lungs initially with a 3% paraformaldehyde/0.1% gluteraldehyde solution (shown in blue). Fixative is delivered to the tracheal catheter at 23 cm H2Opressure (determined by gravity) and to the vascular catheter at 100 cm H2O (determined by positive pressure from a peristaltic pump). Note: use a standard pressure monometer to predetermine the height of the mercury column needed for the pump to generate this pressure. The catheters connect first to a luer adapter, or a luer-adapted needle, and then, via a 4-way stopcock, to the tubing delivering fixative from each reservoir. Hemostat clamps (indicated by black bars) regulate the flow of fixative through the delivery tubes from each reservoir. A gate clip (heavy black bar) positioned on the tubing from the peristaltic pump is used to obtain/regulate pressure in the vascular reservoir.

Article Snippet: 0.28 mm) Luer adapter needles (Beckton-Dickinson for rat trachea and pulmonary artery—16 gauge stub adapter, cat. no. 427561; for mouse trachea, 22G, 0.7 mm × 25.4 mm, cat. no. 305155; and for pulmonary artery, 27G, 0.4 mm × 12.7 mm, cat. no. 305109) 3-way stopcock with male luer-lock adapter (Mallinckrodt, cat. no. 91040) Sterile specimen containers, 4 oz (Fisher Scientific, cat. no. 14-375-147) Single-edged industrial blades (Ted Pella, cat. no. 121-72) Cotton gauze sponges, 2 inch × 2 inch (Fisher Scientific, cat. no. 22-362-178) Kendall Tendersorb underpads, 17 inch × 24 inch (Owens & Minor, cat. no. 3583 007105) Single-use disposable scalpels, no. 15 blade (Fisher Scientific, cat. no. 08-927-5C) Shell vials, 15 mm o.d.

Techniques:

Journal: Molecular imaging and biology

Article Title: 2 nd Window NIR Imaging of Radiation Injury Mitigation Provided by Reduced Notch-Dll4 Expression on Vasculature

doi: 10.1007/s11307-023-01840-7

Figure Lengend Snippet: Study design and number of animals for each experiment

Article Snippet: About ten million lung cells per animal were used for CD31 + enrichment using the CD31 Microbeads kit (cat# 130–109–680, Miltenyi) as per the manufacturer’s instructions.

Techniques: In Vivo, Imaging, Ex Vivo, Expressing, Immunostaining

Journal: Molecular imaging and biology

Article Title: 2 nd Window NIR Imaging of Radiation Injury Mitigation Provided by Reduced Notch-Dll4 Expression on Vasculature

doi: 10.1007/s11307-023-01840-7

Figure Lengend Snippet: SS.BN3 rats have reduced Dll4 expression and reduced susceptibility to late radiation-induced morbidity. a Consomic map illustrating the SS.BN3 rat is generated by the substitution of chromosome 3 from the Brown Norway rat into the Dahl-SS genetic background. b mRNA expression of Dll4 from CD31+ endothelial cells in non-irradiated SS (Dll4-high) and consomic SS.BN3 (Dll4-low) rats (n = 4 (SS) or 5 (SS.BN3) rats per group). c Survival following 13 Gy partial body irradiation (PBI) in adult SS (n = 13) and SS.BN3 rats (n = 19). P value for log-rank analysis: P = 0.0002 for SS vs SS.BN3 following 13 Gy PBI. d Change in body weight relative to pre-irradiation body weight for the rats represented in the survival curve (**P = 0.0066, ****P < 0.0001, two-way ANOVA Sidak’s multiple testing correction). Note the increased drop in body weight at 90 and 120 days in SS as compared to SS.BN3 rats, that occurs during pneumonitis and nephropathy, respectively

Article Snippet: About ten million lung cells per animal were used for CD31 + enrichment using the CD31 Microbeads kit (cat# 130–109–680, Miltenyi) as per the manufacturer’s instructions.

Techniques: Expressing, Generated, Irradiation

Journal: Molecular imaging and biology

Article Title: 2 nd Window NIR Imaging of Radiation Injury Mitigation Provided by Reduced Notch-Dll4 Expression on Vasculature

doi: 10.1007/s11307-023-01840-7

Figure Lengend Snippet: SS.BN3 (Dll4-low) rats have reduced radiation-induced loss of CD31+ endothelial cells. a Left, representative FACS contour plots showing the percentage of CD45−CD31+ lung ECs at day 70 following 13 Gy partial body irradiation in SS (Dll4-high) and SS.BN3 (Dll4-low) rats. Right, quantification of percent CD45−CD31+ cells (n = 9 rats per group). b Left, representative FACS contour plots showing the percentage of CD45−CD31+ kidney ECs at day 70 following 13 Gy partial body irradiation in SS (Dll4-high, n = 4) and SS.BN3 (Dll4-low, n = 5) rats. Right, quantification of percent CD45−CD31+ cells

Article Snippet: About ten million lung cells per animal were used for CD31 + enrichment using the CD31 Microbeads kit (cat# 130–109–680, Miltenyi) as per the manufacturer’s instructions.

Techniques: Irradiation